DNA Evidence in Infidelity Testing: How to Collect, Preserve, and Submit Samples

Collecting evidence for infidelity testing

Collecting, Preserving, and Submiting Samples for Accurate Results

When suspicions of cheating arise, DNA testing can provide scientific evidence to confirm or dismiss concerns. However, obtaining accurate and reliable results depends on proper collection, preservation, and submission of biological samples. Whether you are testing for semen detection, saliva detection, or DNA comparison, following the correct procedures ensures the highest chance of a successful analysis.

Understanding Infidelity DNA Testing

Infidelity DNA testing involves analyzing biological materials found on personal items to determine the presence of semen, saliva, or foreign DNA. The most commonly used infidelity DNA tests include:

  • Semen Detection Tests – Identify traces of semen on clothing, bedding, or personal items.

  • Saliva Detection Tests – Detect saliva residue, which can suggest intimate contact.

  • Advanced DNA Comparison Tests – Compare unknown DNA samples to a provided sample to confirm identity.

  • Complete Infidelity DNA Test – A comprehensive analysis that includes semen detection, saliva detection, and DNA comparison for the most detailed results.

Each of these tests serves a specific purpose, and selecting the right one depends on what you are trying to confirm.

How to Properly Collect Evidence for Infidelity DNA Testing

DNA evidence is highly sensitive, and improper collection can lead to contaminated or degraded samples, resulting in inconclusive results. To ensure accurate testing, follow these best practices when collecting samples:

1. Minimize Handling to Prevent Contamination

✔Always wear gloves when handling fabric, clothing, or other personal items to avoid transferring your own DNA.
✔ Use tweezers or clean tools instead of touching the sample directly.

2. Air-Dry Samples to Prevent DNA Degradation

✔ If the sample contains fluids, such as semen or saliva, allow it to air-dry completely before packaging.
✔ Avoid placing wet samples in sealed containers, as trapped moisture can promote bacterial growth and degrade DNA.

3. Store Samples Correctly for Preservation

Use a paper envelope, not plastic, to store fabric or personal items.
✔ Plastic bags can trap moisture, leading to bacterial contamination and DNA breakdown.
✔ Label each sample with a description, date of collection, and other relevant details.

4. Properly Handle Clothing & Fabric Stains

✔ If testing semen or saliva stains, send the entire fabric or garment whenever possible.
✔ If the item is too large, cut out the stained area—leaving at least an inch of extra fabric around it—to avoid sample loss.

5. Securely Package & Label Personal Items

Toothbrushes, used tissues, and condoms should be placed in separate labeled paper envelopes.
✔ Avoid excessive handling to preserve DNA integrity.
✔ If multiple samples are being sent, keep them in separate envelopes to avoid cross-contamination.

Packaging & Shipping Samples for DNA Testing

To ensure the highest quality results, proper packaging and shipping is crucial:

  1. Compile All Samples – Ensure each item is properly labeled and placed in an individual paper envelope.

  2. Use a Larger Paper Envelope or Box – Place all individual envelopes in a larger shipping envelope or a sturdy box for added protection.

  3. Choose a Reliable Shipping Method – Use USPS, FedEx, or UPS for trackable and timely delivery to the laboratory.

  4. Avoid Extreme Temperatures – Store and ship samples in a cool, dry place to prevent DNA degradation.

  5. Notify the Lab of Special Samples – If submitting forensic DNA samples (toothbrushes, hair, or personal items), inform the lab beforehand for proper handling instructions.

How Long Does DNA Last on Items?

DNA stability depends on storage conditions, material type, and exposure. Here’s how long DNA typically lasts on different items:

  • Fabric (Clothing, Sheets, Underwear): DNA can remain for months to years if stored in a cool, dry place.

  • Toothbrushes & Gum: DNA can last weeks to months, but bacterial growth may degrade it over time.

  • Tissues, Napkins, Condoms: DNA deteriorates faster if exposed to moisture or heat, lasting days to weeks.

  • Hair with Root Attached: Can contain viable DNA for years if properly stored.

While DNA is relatively stable, heat, humidity, and UV light can rapidly degrade samples, making timely collection and storage crucial.

Ensuring Accurate Infidelity DNA Test Results

Proper collection, storage, and shipping are essential for accurate and reliable results in infidelity DNA testing. To maximize your chances of obtaining a conclusive result, follow these key takeaways:

Use gloves and tools to handle items to avoid contamination.
Let wet samples air-dry completely before storage.
Store samples in paper envelopes, never plastic.
Send samples as soon as possible for the highest accuracy.
Choose the right test—semen detection, saliva testing, or DNA comparison—for your situation.

At Validity Genetics, we provide accurate, discreet, and scientifically validated infidelity DNA testing to help you uncover the truth. Order your test today and get the clarity you need.